Molecular Cellbiology - Zebrafish demonstration movies
- Time lapse of a zebrafish cell grown in culture.
Cells of cell line ZF13 were transfected with a plasmid expressing a GFP-actin fusion protein and monitored with confocal laser scanning microscopy. More details can be found in the following paper: Breek, C. K. D., F. van Iren, S. E. Wijting, N. Stuurman, and H. P. Spaink. 2002. Expression of multicolor fluorescent fusion proteins in zebrafish cell cultures: a versatile tool in cell biology, p. 373-379. In: Kraayenhof, Visser, and Gerritsen (eds.), Fluorescence Spectroscopy, Imaging and Probes. Springer Verlag, Heidelberg.
- Time lapse of zebrafish embryo during 18 hrs of development.
(by Rolf Karlstrom and Don Kane; Development 123:461, 1966). This movie is taken from the zfin web page
- Time lapse movie of zebrafish cells using the fluorescent calcium dye fluo-4.
(Author, Nico Stuurman, Institute of Biology Leiden). Addition of epidermal growth factor to these cells leads to a strong increase in the cytosolic calcium concentration.